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Calmodulin,CAM anticorps (AA 1-149)

Reactivité: Poulet WB, ICC, ELISA, IHC (fro), IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1077888
  • Antigène Voir toutes Calmodulin,CAM Anticorps
    Calmodulin,CAM
    Épitope
    AA 1-149
    Reactivité
    • 2
    • 1
    Poulet
    Hôte
    • 3
    Lapin
    Clonalité
    • 3
    Polyclonal
    Conjugué
    • 1
    • 1
    • 1
    Cet anticorp Calmodulin,CAM est non-conjugé
    Application
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Séquence
    MGHHHHHHSG S-MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
    Specificité
    It has been selected for its ability to recognize CAM in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogène
    Recombinant CAM expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against CAM.
    Isotype
    IgG
    Top Product
    Discover our top product Calmodulin,CAM Anticorps primaire
  • Indications d'application
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Commentaires

    Content: The quality control contains recombinant CAM (Met1~Lys149) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Date de péremption
    12 months
  • Antigène
    Calmodulin,CAM
    Autre désignation
    Calmodulin (CAM) (Calmodulin,CAM Produits)
    Synonymes
    anticorps CALM2, anticorps CaMA, anticorps R75142, anticorps PHKD, anticorps PHKD3, anticorps calmodulin 1, anticorps calmodulin 2, anticorps calmodulin 3, anticorps CALM1, anticorps Calm2, anticorps CALM2, anticorps Calm3, anticorps CALM3
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