Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.
Pureté
Anti-TRYPSINOGEN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].
niveau d'endotoxine
Low Endotoxin : No
Immunogène
Trypsinogen [Bovine Pancreas] Immunogen Type: Native Protein
Reactivité: Boeuf (Vache)
WB, ELISA, IP, DB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
TRYPSINOGEN Antibody has been assayed against 1.0 µg of Trypsinogen [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,300 to 1:8,400 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:25.000 Western Blot Dilution: 1:500 - 1:3.000
Restrictions
For Research Use only
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Conseil sur la manipulation
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Date de péremption
Expiration date is one (1) year from date of opening.