VSV-g Tag anticorps

N° du produit ABIN108207

Détail du produit anti-VSV-g Tag anticorps

Antigène: VSV-g Tag (VSV-G Epitope Tag)

Reactivité: Vesicular Stomatitis Virus (VSV)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VSV-g Tag est non-conjugé

Application: ELISA, Fluorescence Microscopy (FM)

Séquence: YTDIEMNRLG K

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluor-escence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200.   At higher concentrations, a filamentous background staining may be present.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.17 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-VSV-g Tag (ABIN108207)

Muñoz-Álvarez, Altomonte, Laitinen, Ziegler, Steiger, Esposito, Schmid, Ebert: "PET imaging of oncolytic VSV expressing the mutant HSV-1 thymidine kinase transgene in a preclinical HCC rat model." dans: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 4, pp. 728-36, (2016) (PubMed).

Détails sur VSV-g Tag

Antigène: VSV-g Tag (VSV-G Epitope Tag)

Autre désignation: VSV-G Tag (VSV-g Tag Produits)

Classe de substances: Tag

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA.  VSV-G or vesicular stomatitis virus glycoprotein is found within the pseudo lentiviral cloning vector pHCMV-VSV-G.