PABP anticorps

N° du produit ABIN108538

Cet anticorps Souris Monoclonal détecte spécifiquement PABP dans WB, ELISA, IF, IP, FACS et ICC. Il présente une réactivité avec des échantillons de Humain, Lapin, Poulet, Xenopus laevis et Hamster. Il a été cité dans 3+ publications.

Aperçu rapide pour PABP anticorps (ABIN108538)

Antigène: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Reactivité: Humain, Lapin, Poulet, Xenopus laevis, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PABP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 10E10

Détail du produit anti-PABP anticorps

Purification: Protein G

Immunogène: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI

Isotype: IgG2b

Information d'application

Indications d'application: Optimal antibody dilution should be determined by titration, however try:WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa)ICC/IF: Use at an assay dependent dilution. PubMed: 17977970Flow Cyt: Use 1 μg for 106 cells

Commentaires:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-PABP (ABIN108538)

Gorgoni, Richardson, Burgess, Anderson, Wilkie, Gautier, Martins, Brook, Sheets, Gray: "Poly(A)-binding proteins are functionally distinct and have essential roles during vertebrate development." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 19, pp. 7844-9, (2011) (PubMed).

Unsworth, Raguz, Edwards, Higgins, Yagüe: "mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 9, pp. 3370-80, (2010) (PubMed).

Görlach, Burd, Dreyfuss: "The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity." dans: Experimental cell research, Vol. 211, Issue 2, pp. 400-7, (1994) (PubMed).

Détails sur PABP

Antigène: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Autre désignation: PABP

Sujet: Synonyms:
PAB 1 antibody,PAB1 antibody,PABP 1 antibody,PABP1 antibody,PABPC 1 antibody,PABPC1antibody,PABPC2 antibody,PABPL1 antibody,Poly A binding protein 1 antibody,Poly A binding protein cytoplasmic1 antibody,Polyadenylate binding protein 1 antibody

ID gène: 117282

OMIM: 604679

UniProt: P22626

Pathways: SARS-CoV-2 Protein Interactome