Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)
Specificité
The antibody reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored glycoprotein expressed on various cell types including erythrocytes, lymphoblasts, at low levels it is present on circulating lymphocytes.
SEMA7A
Reactivité: Humain
ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow Cytometry. Immunoprecipitation. Western Blotting under non reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors plexin C1 and b1 integrins.Synonyms: JMH blood group antigen, John-Milton-Hargen human blood group Ag, SEMAL, Semaphorin-7A, Semaphorin-K1, Semaphorin-L