Reactivité: Souris
FACS, IHC (fro)
Hôte: Rat
unconjugated
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at < -20 °C for at least 6 months.
Date de péremption
6 months
Antigène
MHC Class II Antigen I Ak,d,b,q,r
Autre désignation
MHC Class II (I-A K,b,d,q,r)
Sujet
MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II.