Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7
Réactivité croisée (Details)
Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish
Purification
Protein A chromatography
Immunogène
Human keratin K18 from HeLa cytoskeletal preparation
Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 1.0 mL distilled water.
Buffer
PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing). Dilute only prior to immediate use.
Stock
-20 °C
Stockage commentaire
Store the antiserum at -20 °C (aliquot contents to
Zatloukal, Kufferath, Thueringer, Landegren, Zatloukal, Haybaeck: "Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies." dans: PLoS ONE, Vol. 9, Issue 5, pp. e96690, (2014) (PubMed).
Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." dans: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, (2012) (PubMed).
Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).
Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." dans: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).
Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." dans: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, (2003) (PubMed).
Schaffeld, Knappe, Hunzinger, Markl: "cDNA sequences of the authentic keratins 8 and 18 in zebrafish." dans: Differentiation; research in biological diversity, Vol. 71, Issue 1, pp. 73-82, (2003) (PubMed).
Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." dans: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, (2002) (PubMed).
Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." dans: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, (2001) (PubMed).
Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." dans: Neuron, Vol. 14, Issue 1, pp. 29-41, (1995) (PubMed).
Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." dans: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).
Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).
Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." dans: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).
Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." dans: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).
anticorps CK18, anticorps K18, anticorps Krt1-18, anticorps CYK18, anticorps DreK18, anticorps cb83, anticorps dapk1, anticorps sb:cb83, anticorps wu:fa13f02, anticorps wu:fb36b04, anticorps krt18, anticorps MGC64569, anticorps MGC75922, anticorps KRT18, anticorps CK-18-A, anticorps K18-A, anticorps NC-11, anticorps cyk18, anticorps k18, anticorps krt18-a, anticorps krt18-b, anticorps krt18a, anticorps xkendob, anticorps keratin 18, anticorps keratin 18, type I L homeolog, anticorps keratin 18, type I, anticorps keratin, type I cytoskeletal 18, anticorps K18, simple type I keratin, anticorps Keratin, type I cytoskeletal 18, anticorps keratin 18, type I S homeolog, anticorps Krt18, anticorps KRT18, anticorps krt18, anticorps krt18.L, anticorps LOC700569, anticorps LOC100136778, anticorps k1c18, anticorps LOC100008885, anticorps krt18.S
Sujet
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18