ITGA3 anticorps (PE)

N° du produit ABIN1112174

Détail du produit anti-ITGA3 anticorps (PE)

Antigène: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivité: Humain

Hôte: Veuillez nous consulter SVP

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA3 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: CD49c, a 150kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is expressed by most cell types including T and B lymphocytes

Clone: VJ1-6

Isotype: IgG1

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).

Préparation de l'échantillon:

1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD49c PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer.8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Détails sur ITGA3

Antigène: ITGA3 (Integrin, alpha 3 (ITGA3))

Autre désignation: CD49c (ITGA3 Produits)

Synonymes: anticorps CD49C, anticorps GAP-B3, anticorps GAPB3, anticorps ILNEB, anticorps MSK18, anticorps VCA-2, anticorps VL3A, anticorps VLA3a, anticorps ITGA3, anticorps AA407068, anticorps integrin subunit alpha 3, anticorps integrin subunit alpha 3 S homeolog, anticorps integrin alpha 3, anticorps ITGA3, anticorps itga3.S, anticorps Itga3

Sujet: The antibody recognizes the VLA-a3 chain of the VLA-3 complex and is reactive with epithelial tissue and basal renal distal tubules.

Pathways: CXCR4-mediated Signaling Events, Integrin Complex