CD53 anticorps (APC)

N° du produit ABIN1112183

Détail du produit anti-CD53 anticorps (APC)

Antigène: CD53

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD53 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Monoclonal Mouse Anti-Human CD53.The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA),(Lote: 113K1364/SIGMA) and 0,09% NaN3, pH 7.2.

Clone: HI29

Isotype: IgG1

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

Allophycocyanin (Febico, Far East Bio-Tech Co.).

Préparation de l'échantillon:

1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD53 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Références pour anticorps anti-CD53 (APC) (ABIN1112183)

Kim, Yoon, Kim, Yook, Kim, Kim, Lee, Paik: "LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress." dans: Molecules and cells, Vol. 17, Issue 1, pp. 125-31, (2004) (PubMed).

Yunta, Rodríguez-Barbero, Arévalo, López-Novoa, Lazo: "Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells." dans: Kidney international, Vol. 63, Issue 2, pp. 534-42, (2003) (PubMed).

Détails sur CD53

Antigène: CD53

Autre désignation: CD53 (CD53 Produits)

Synonymes: anticorps AI323659, anticorps Ox-44, anticorps Tspan25, anticorps MOX44, anticorps TSPAN25, anticorps OX44, anticorps CD53 antigen, anticorps CD53 molecule, anticorps Cd53 molecule, anticorps Cd53, anticorps CD53

Sujet: The antigen, 32-40kDa, is a single chain glycoprotein on most leucocytes. There is no cross- reactivity with erythrocytes or platelets. The antibody recognises a broad span of leucocytes of both normal and neoplastic plasma cells.