CD8 anticorps (APC)

N° du produit ABIN1112229

Détail du produit anti-CD8 anticorps (APC)

Antigène: CD8

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Monoclonal Mouse Anti-Human CD8 APC, is recommended for use in flow cytometry for identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen.

Clone: MEM-31

Isotype: IgG2a

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

Allophycocyanin (Febico, Far East Bio-Tech Co.).

Prélèvement de l'échantillon: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD8 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Références pour anticorps anti-CD8 (APC) (ABIN1112229)

Lyle, Christofidou-Solomidou, Liu, Elder, Albelda, Cotsarelis: "Human hair follicle bulge cells are biochemically distinct and possess an epithelial stem cell phenotype." dans: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 4, Issue 3, pp. 296-301, (2000) (PubMed).

Nuckols, Shea, Horenstein, Burchette, Prieto: "Quantitation of intraepidermal T-cell subsets in formalin-fixed, paraffin-embedded tissue helps in the diagnosis of mycosis fungoides." dans: Journal of cutaneous pathology, Vol. 26, Issue 4, pp. 169-75, (1999) (PubMed).

Yamagata, Tanaka, Kudo: "A quantitative immunohistochemical evaluation of inflammatory cells at the affected and unaffected sites of inflammatory bowel disease." dans: Journal of gastroenterology and hepatology, Vol. 13, Issue 8, pp. 801-8, (1998) (PubMed).

Détails sur CD8

Antigène: CD8

Autre désignation: CD8 (CD8 Produits)

Synonymes: anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps CD8a molecule, anticorps CD8A

Sujet: 30/32 kDa MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.