CD40 anticorps

N° du produit ABIN111321

Détail du produit anti-CD40 anticorps

Antigène: CD40

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: CD40 B cells.

Immunogène: Anti-mu activated tonsillar human B cells.

Clone: MAB89

Isotype: IgG1

Information d'application

Indications d'application: Fluorescent Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Activation assays: Working concentration: 10 ng/mL. Study of of B-cell proliferation, B-cell growth and activation factors. Functional Study of B-cell subpopulations.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL of distilled water.

Concentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS buffer containing 1 mg/mL BSA as stabilizer without preservatives.

Agent conservateur: Without preservative

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur CD40

Antigène: CD40

Autre désignation: CD40 (CD40 Produits)

Sujet: CD40 is a 49 kDa type I integral membrane protein of the NGF receptor family. CD40 is found on normal and neoplastic B cells.(1) It is a pan-B marker, absent only from terminally differentiated plasma cells. CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that it induces strong homotypic adhesions in resting B cells and, together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. (1,2) Purified B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. (3) The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. (4) This clone acts in synergy with IL-4 to induce the increase of B-cell volume and the proliferation of anti IgM antibody-co-stimulated or pre-activated B cells. (5)Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

ID gène: 958

NCBI Accession: NP_001241

UniProt: P25942

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints