Species reactivity (tested):Mouse (Cells expressing MHC class II antigens).
Purification
Affinity Chromatography
Immunogène
Murine thymic reticulum Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.
Reactivité: Souris
FACS, IHC (fro)
Hôte: Rat
unconjugated
Indications d'application
Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 0.5 mL distilled water.
Concentration
0.4 mg/mL
Buffer
Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer with no preservative
Agent conservateur
Without preservative
Conseil sur la manipulation
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Stock
4 °C
Stockage commentaire
Store at +4 °C. DO NOT FREEZE. This product should be stored undiluted.
Antigène
MHC Class II Antigen I Ak,d,b,q,r
Autre désignation
MHC Class II (I-A K,b,d,q,r)
Sujet
MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.