Reactivité: Souris
IHC (fro)
Hôte: Rat
Monoclonal
MIDC-8
unconjugated
Indications d'application
Immunohistochemistry on frozen sections: use at 0.5 μg/mL (1: 400). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocole
Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
Restrictions
For Research Use only
Reconstitution
Restore with 0.5 mL distilled water.
Concentration
0.2 mg/mL
Buffer
PBS buffer with 0.02 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Antigène
Dendritic Cells
Sujet
Monoclonal antibody Ki-M4 detects the accessory B-cell macrophages in lymphoid organs.Synonyms: Dendritic cell marker