Mitotic Cells anticorps
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- Antigène
- Mitotic Cells
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Reactivité
- Humain, Poisson zèbre (Danio rerio)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Inconjugué
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- Réactivité croisée (Details)
- Species reactivity (tested):Human, Zebrafish.
- Purification
- Immunoaffinity Chromatography
- Immunogène
- Total cell lysate of the human bladder carcinoma cell line T24.
- Clone
- 8B3G
- Isotype
- IgM
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- Indications d'application
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This antibody is suitable for Flow cytometric analyses (1/50-1/100) andImmunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent (1/50-1/100). Not suitable for Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the lyophilized antibody at 2-8 °C for up to one month or at -20 °C for longer.
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- Antigène
- Mitotic Cells
- Sujet
- The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase. M phase itself is highly regulated, and is divided into five stages: prophase, prometaphase, metaphase, telophase and anaphase.
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