Ly6g anticorps
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- Antigène Tous les produits Ly6g
- Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Ly6g est non-conjugé
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Cytotoxicity Test (CyTox)
- Purification
- Protein G Chromatography.
- Clone
- RB6-8C5
- Isotype
- IgG2b
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- Indications d'application
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This antibody is suitable for studies of myeloid differentiation stages and their regulationsby cytokines. Applications include Flow Cytometry (1,2,3) complement-mediated depletion(4), Western blot staining (5) and both Frozen and Paraffin Sections (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of ABIN114273. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) . 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsIsotypic Control: Rat IgG2bCell Source-Percentage of cells stained above control: Thymus: 2. 0%Whole Blood Monocytes: 79. 1%Bone Marrow Macrophages: 87. 5%N. B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigatordetermine dilutions appropriate for individual use. Strain Distribution by Flow Cytometry Analysis: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsStrains Tested: BALB/c, C57BL/6, CBA, C3H/He, AKR
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS and 0.02 % Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
- Autre désignation
- Ly6G / GR1 Neutrophil Marker (Ly6g Produits)
- Synonymes
- anticorps Gr-1, anticorps Gr1, anticorps Ly-6G, anticorps lymphocyte antigen 6 complex, locus G, anticorps Ly6g
- Sujet
- Synonyms: Gr-1 Granulocyte marker
- ID gène
- 10090
- UniProt
- P35461
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