MHC Class II I-Ap anticorps

N° du produit ABIN114314

L’anticorps anti-MHC Class II I-Ap Monoclonal Souris est utilisé pour la détection de MHC Class II I-Ap dans des échantillons de Souris. Il a été validé pour FACS et CyTox.

Aperçu rapide pour MHC Class II I-Ap anticorps (ABIN114314)

Antigène: MHC Class II I-Ap

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox)

Clone: 7-16-17

Détail du produit

Purification: Protein G chromatography

Immunogène: B10.p

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry (protocol see below). Cytotoxic assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS and 0.02 % sodium azide (NaN3).

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: MHC Class II I-Ap