This antibody is specific to a protein of 110 kDa, identified as androgen receptor. This antibody reacts with full length of AR and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors. Cellular Localization: Nuclear. Has been reported to cross-react with Dog.
Purification
Purified
Immunogène
BALB/C mice were injected with a synthetic peptide, amino acid 340-356 (STEDTAEYSPFKGGYTK) of Human Androgen Receptor
AR
Reactivité: Humain, Souris, Rat
IHC (p), IF (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot: 1: 50 to 1: 100. Immunohistochemistry on paraffin embedded sections: dilute 1: 25-1: 50 in an ABC method(30 minutes at room temperature). Formalin fixed paraffin embedded tissue sectionsrequire high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior toimmunostaining. Recommended Positive Control: Prostate carcinoma Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.Synonyms: DHTR, Dihydrotestosterone receptor, NR3C4, Nuclear receptor subfamily 3 group C member 4