Periostin anticorps
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- Antigène Voir toutes Periostin (POSTN) Anticorps
- Periostin (POSTN)
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Reactivité
- Humain, Souris, Rat, Poulet
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Periostin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Séquence
- MGHHHHHHHH HHSSGHIEGR HMRNNHYDKI LAHSRIRGRD QGPNVCALQQ ILGTKKKYFS TCKNWYKKSI CGQKTTVLYE CCPGYMRMEG MKGCPAVLPI DHVYGTLGIV GATTTQRYSD ASKLREEIEG KGSFTYFAPS NEAWDNLDSD IRRGLESNVN VELLNALHSH MINKRMLTKD LKNGMIIPSM YNNLGLFINH YPNGVVTVNC ARIIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFIEAEDDLS SFRAAAITSD ILEALGRDGH FTLFAPTNEA FEKLPRGVLE RFMGDKVASE ALMKYHILNT LQCSESIMGG AVFETLEGNT IEIGCDGDSI TVNGIKMVNK KDIVTNNGVI HLIDQVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASALR PDGEYTLLAP VNNAFSDDTL SMVQRLLKLI LQNHILKVKV GLNELYNGQI LETIGGKQLR VFVYRTAVCI ENSCMEKGSK QGRNGAIHIF REIIKPAEKS LHEKLKQDKR FSTFLSLLEA ADLKELLTQP GDWTLFVPTN DAFKGMTSEE KEILIRDKNA LQNIILYHLT PGVFIGKGFE PGVTNILKTT QGSKIFLKEV NDTLLVNELK SKESDIMTTN GVIHVVDKLL YPADTPVGND QLLEILNKLI KYIQIKFVRG STFKEIPVTV Y Remarks: The immunizati on antigen (75 kDa) is a protein containing 648 amino acids of recombinan t Human OSF-2 and 23 amino acids, N-Terminal HisTag and Xa - cleavage site (highlighte d). The antigen contains amino acid residues 22 to 669 of the Human OSF-2 precursor. The antigen includes all four fasciclin domains of OSF-2.
- Specificité
- The antibody recognizes OSF-2.
- Purification
- Immunoaffinity Chromatography on Immoblized Recombinant Human OSF2
- Immunogène
- Recombinant Human OSF-2 Source of Antigen: E. coli.
- Top Product
- Discover our top product POSTN Anticorps primaire
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- Indications d'application
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ELISA. Western blot (0.1-1 μg/mL). Immunohistochemistry on Paraffin (1/100-1/5,000) and Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
- Buffer
- 0.05 M Phosphate Buffer, 0.1 M NaCl, pH 7.2 without preservatives
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Stable the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- Periostin (POSTN)
- Autre désignation
- Periostin (POSTN Produits)
- Synonymes
- anticorps fa99h07, anticorps pn, anticorps postn, anticorps wu:fa99h07, anticorps wu:fc70f09, anticorps zgc:153873, anticorps LOC100008945, anticorps osf-2, anticorps pdlpostn, anticorps periostin, anticorps OSF-2, anticorps OSF2, anticorps PDLPOSTN, anticorps PN, anticorps RP11-412K4.1, anticorps A630052E07Rik, anticorps AI747096, anticorps Osf2, anticorps PLF, anticorps peri, anticorps Plf, anticorps periostin, anticorps periostin, osteoblast specific factor b, anticorps periostin, osteoblast specific factor, anticorps POSTN, anticorps postnb, anticorps postn, anticorps Postn
- Sujet
- Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN
- ID gène
- 10631
- NCBI Accession
- NP_001129406
- UniProt
- Q15063
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