CCL16
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman LEC (100 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1-2 μg/mL of thisantibody will detect at least 0.03 ng/well of recombinant human LEC when used withbiotinylated antigen affinity purified anti-human LEC as the detection antibody at aconcentration of at least 1 μg/mL. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at aconcentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 1.0 mg/mL.
Buffer
PBS without preservatives
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.Synonyms: C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, ILINCK, LCC-1, LCC1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, SCYA16