IL1A anticorps (Biotin)
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- Antigène Voir toutes IL1A Anticorps
- IL1A (Interleukin 1 alpha (IL1A))
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Reactivité
- Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL1A est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- This antibody reacts with Mouse Interleukin-1 alpha (IL-1 alpha).
- Purification
- Affinity chromatography
- Immunogène
- Highly pure (>98%) recombinant mIL-1 alpha
- Top Product
- Discover our top product IL1A Anticorps primaire
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- Indications d'application
- ELISA: (Direct): To detect mIL-1α by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mIL-1α. (Sandwich): To detect mIL-1α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine IL-1α (PP005P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1α. Western blot: To detect mIL-1α by Western blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing
- Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Antigène
- IL1A (Interleukin 1 alpha (IL1A))
- Autre désignation
- Interleukin-1 alpha / IL-1A (IL1A Produits)
- Synonymes
- anticorps IL-1A, anticorps IL1, anticorps IL1-ALPHA, anticorps IL1F1, anticorps IL-6, anticorps Il-1a, anticorps IL-1 alpha, anticorps IL-1alpha, anticorps L1A, anticorps interleukin 1 alpha, anticorps interleukin 6, anticorps IL1A, anticorps IL6, anticorps Il1a
- Sujet
- Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
- ID gène
- 16175
- NCBI Accession
- NP_034684
- UniProt
- P01582
- Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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