Ccl12
Reactivité: Souris
ELISA, WB
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
ELISA: To detect mMCP-5 by direct ELISA (using 100 l/well antibody solution) this antibody canbe used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations.Synonyms: C-C motif chemokine 12. Macrophage Chemotactic Protein-5, CCL-12, MCP-1-related chemokine, MCP5, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12