CTF1
Reactivité: Humain
WB, IHC, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhCardiotrophin-1 (3 ng/mL), a concentration of 0.04-0.09 μg/mL of this antibody isrequired. ELISA: To detect hCardiotrophin-1 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hCardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCardiotrophin-1 is 1.5-3.0 ng/lane, underreducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Cardiotrophin-1 (CT-1), a cardiac hypertrophic factor, is a 21.5 kDa protein member of the IL-6 cytokine family. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. In addition, CT-1 activates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes and enhances transcription factor NF kappa B DNA -binding activities. CT-1 is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine.Synonyms: CT-1, CTF1