CCL21
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman Exodus-2 (100.0 ng/mL), a concentration of 6.0-8.0 μg/mL of this antibody isrequired. ELISAIndirect: To detect Human Exodus-2 by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human Exodus-2. Sandwich: To detect Human Exodus-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human Exodus-2 (PP1005B1 orPP1005B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human Exodus-2. Western Blot: To detect Human Exodus-2 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human Exodus-2 is 1.5-3.0 ng/lane, under
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Centrifuge vial prior to opening! This product is photosensitive and should be protected from light
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).Synonyms: Beta chemokine Exodus-2, CCL-21, SCYA21, SLC, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21