CCL1 anticorps (Biotin)
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- Antigène Voir toutes CCL1 Anticorps
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CCL1 est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- Recognizes Human I-309
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatography.
- Immunogène
- Highly pure (>98%) recombinant Human I-309. Remarks: Molecular Weight: ~150 kDa
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- Discover our top product CCL1 Anticorps primaire
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- Indications d'application
- Direct ELISA: To detect Human I-309 by Direct ELISA (using 100 μL/well) a concentration ofapprox. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonal antibody allows thedetection of at least 0.2-0.4 ng/well of recombinant Human I-309. Sandwich ELISA: To detect Human I-309 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody, in conjunction with anti-Human I-309 (PP1017P1 or PP1017P2) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human I-309. Western Blot: To detect Human I-309 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human I-309 is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Antigène
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
- Autre désignation
- CCL1 / I-309 (CCL1 Produits)
- Synonymes
- anticorps I-309, anticorps P500, anticorps SCYA1, anticorps SISe, anticorps TCA3, anticorps BF534335, anticorps Scya1, anticorps Tca-3, anticorps CCLi6, anticorps chemokine, anticorps C-C motif chemokine ligand 1, anticorps chemokine (C-C motif) ligand 1, anticorps CCL1, anticorps Ccl1
- Sujet
- I-309 is a member of the C-C, or beta chemokine class. Other chemokines in this group include MIP1 alpha, MIP1 beta, RANTES, MCP1/2/3/4, mouse JE, 1 - 2 μg/mL antibody detects 20 and mouse MARC, eotaxin, HCC1 and C10. These chemokines act primarily as chemoattractants and activate monocytes, dendritic cells, T lymphocytes, natural killer cells, B lymphocytes, basophils and eosinophils. I-309 was originally identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line but not in EBVtransformed B cells. I-309 is a chemoattractant for monocytes, but not for neutrophils. IP-10 is an approximately 8.5 kD polypeptide of 73 amino acids. The precursor form of human I-309 consists of 96 amino acids. To generate the mature I-309, the precursor cleaves its hydrophobic signal peptide. Human I-309 is thought to be a homologue of the mouse TCA3, and shows 42 % amino acid homology to mouse TCA3.Synonyms: C-C motif chemokine 1, CCL-1, SCYA1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
- ID gène
- 6346
- NCBI Accession
- NP_002972
- UniProt
- P22362
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