CCL8 anticorps
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- Antigène Voir toutes CCL8 Anticorps
- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CCL8 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Specificité
- Human Macrophage Chemotactic Protein-2 (MCP-2).
- Purification
- Affinity chromatography.
- Immunogène
- Highly pure (>98%) recombinant hMCP-2.
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- Discover our top product CCL8 Anticorps primaire
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- Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-2 (100.00 ng/mL), a concentration of 12-16 μg/mL of this antibody is required. ELISA: To detect hMCP-2 by Indirect ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5-0.2 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMCP-2. Sandwich: To detect hMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MCP-2 (PP1045B1/B2) as adetection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinanthMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Centrifuge vial prior to opening!
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
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- Antigène
- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
- Autre désignation
- MCP2 / CCL8 (CCL8 Produits)
- Synonymes
- anticorps HC14, anticorps MCP-2, anticorps MCP2, anticorps SCYA10, anticorps SCYA8, anticorps 1810063B20Rik, anticorps AB023418, anticorps Mcp2, anticorps Scya8, anticorps C-C motif chemokine ligand 8, anticorps chemokine (C-C motif) ligand 8, anticorps C-C motif chemokine 8, anticorps monocyte chemoattractant protein-2 precursor, anticorps CCL8, anticorps Ccl8, anticorps LOC101119572, anticorps MCP-2
- Sujet
- MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8
- ID gène
- 6355
- NCBI Accession
- NP_005614
- UniProt
- P80075
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