OSM
Reactivité: Humain
WB, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: To detect hOncostatin M by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthOncostatin M. Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hOncostatin M is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.