Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificité
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
Purification
Prepared from monospecific antiserum by a delipidation and defibrination.
RNASE1
Reactivité: Souris
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended dilutions: ELISA: 1/30,000-1/150,000. Western blot: 1/3,000-1/15,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with no preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Antigène
RNASE1
(Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))