Caspase 8 anticorps (AA 181-478)
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- Antigène Voir toutes Caspase 8 (CASP8) Anticorps
- Caspase 8 (CASP8)
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Épitope
- AA 181-478
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Caspase 8 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificité
- Recognizes the p18 subunit of human caspase-8.
- Réactivité croisée
- Humain
- Purification
- Purified from concentrated hybridoma tissue culture supernatant.
- Pureté
- >95 % (SDS-PAGE)
- Immunogène
- Recombinant human caspase-8 (aa 181-478).
- Clone
- C15
- Isotype
- IgG2b
- Top Product
- Discover our top product CASP8 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - Date de péremption
- 12 months
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Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation." dans: Molecular and cellular biology, Vol. 23, Issue 3, pp. 777-90, (2003) (PubMed).
: "Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8." dans: The EMBO journal, Vol. 21, Issue 17, pp. 4520-30, (2002) (PubMed).
: "Involvement of caspases and of mitochondria in Fas ligation-induced eosinophil apoptosis: modulation by interleukin-5 and interferon-gamma." dans: Journal of leukocyte biology, Vol. 70, Issue 5, pp. 767-75, (2001) (PubMed).
: "FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2." dans: Immunity, Vol. 12, Issue 6, pp. 599-609, (2000) (PubMed).
: "Liposomal ET-18-OCH(3) induces cytochrome c-mediated apoptosis independently of CD95 (APO-1/Fas) signaling." dans: Blood, Vol. 94, Issue 10, pp. 3583-92, (1999) (PubMed).
: "Bcl-xL acts downstream of caspase-8 activation by the CD95 death-inducing signaling complex." dans: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3388-93, (1998) (PubMed).
: "Two CD95 (APO-1/Fas) signaling pathways." dans: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, (1998) (PubMed).
: "FLICE is predominantly expressed as two functionally active isoforms, caspase-8/a and caspase-8/b." dans: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 26953-8, (1997) (PubMed).
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Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation." dans: Molecular and cellular biology, Vol. 23, Issue 3, pp. 777-90, (2003) (PubMed).
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- Antigène
- Caspase 8 (CASP8)
- Autre désignation
- Caspase-8 (CASP8 Produits)
- Synonymes
- anticorps casp8-A, anticorps xCaspase-8, anticorps casp8, anticorps CASP8, anticorps CASP-8, anticorps FLICE, anticorps MACH, anticorps Mch5, anticorps ALPS2B, anticorps CAP4, anticorps Casp-8, anticorps MCH5, anticorps caspase-8, anticorps zgc:92075, anticorps caspase 8 L homeolog, anticorps death related ced-3/Nedd2-like protein, anticorps caspase 8, anticorps caspase-8, anticorps caspase-8-like, anticorps caspase 8, apoptosis-related cysteine peptidase, anticorps casp8.L, anticorps LOC661095, anticorps CASP8, anticorps LOC5563818, anticorps casp8, anticorps LOC100222284, anticorps Casp8
- Sujet
- Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p18, respectively, which leads to the generation of the active caspase-8 heterotetramer (p18/p10)2.
- UniProt
- Q14790
- Pathways
- Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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