MAVS anticorps (AA 160-450)

N° du produit ABIN1169132

Détail du produit anti-MAVS anticorps

Antigène: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Épitope: AA 160-450

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAVS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Recognizes human Cardif.

Réactivité croisée: Humain

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Recombinant human Cardif (aa 160-450).

Clone: Adri-1

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Références pour anticorps anti-MAVS (ABIN1169132)

Morikawa, Gouttenoire, Hernandez, Dao Thi, Tran, Lange, Dill, Heim, Donzé, Penin, Quadroni, Moradpour: "Quantitative proteomics identifies the membrane-associated peroxidase GPx8 as a cellular substrate of the hepatitis C virus NS3-4A protease." dans: Hepatology (Baltimore, Md.), Vol. 59, Issue 2, pp. 423-33, (2014) (PubMed).

Bellecave, Sarasin-Filipowicz, Donzé, Kennel, Gouttenoire, Meylan, Terracciano, Tschopp, Sarrazin, Berg, Moradpour, Heim: "Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system." dans: Hepatology (Baltimore, Md.), Vol. 51, Issue 4, pp. 1127-36, (2010) (PubMed).

Michallet, Meylan, Ermolaeva, Vazquez, Rebsamen, Curran, Poeck, Bscheider, Hartmann, König, Kalinke, Pasparakis, Tschopp: "TRADD protein is an essential component of the RIG-like helicase antiviral pathway." dans: Immunity, Vol. 28, Issue 5, pp. 651-61, (2008) (PubMed).

Détails sur MAVS

Antigène: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Autre désignation: Cardif (MAVS Produits)

Synonymes: anticorps CARDIF, anticorps IPS-1, anticorps IPS1, anticorps VISA, anticorps D430028G21Rik, anticorps Visa, anticorps cardif, anticorps wu:fj20d04, anticorps zgc:158392, anticorps mitochondrial antiviral signaling protein, anticorps MAVS, anticorps Mavs, anticorps mavs

Sujet: RIG-I (retinoic acid-inducible gene I, Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

UniProt: Q7Z434

Pathways: Activation of Innate immune Response, Inositol Metabolic Process, Hepatitis C