HCV 1b NS5B anticorps

N° du produit ABIN1169141

Détail du produit anti-HCV 1b NS5B anticorps

Antigène: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Reactivité: Hepatitis C Virus (HCV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HCV 1b NS5B est non-conjugé

Application: Western Blotting (WB)

Specificité: Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity).

Réactivité croisée: Hepatitis C Virus (HCV)

Réactivité croisée (Details): It does not recognize genotype 2a.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).

Clone: 5B-3B1

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Références pour anticorps anti-HCV 1b NS5B (ABIN1169141)

Kapadia, Chisari: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).

Quinkert, Bartenschlager, Lohmann: "Quantitative analysis of the hepatitis C virus replication complex." dans: Journal of virology, Vol. 79, Issue 21, pp. 13594-605, (2005) (PubMed).

Svitkin, Pause, Lopez-Lastra, Perreault, Sonenberg: "Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3." dans: Journal of virology, Vol. 79, Issue 11, pp. 6868-81, (2005) (PubMed).

Hardy, Marcotrigiano, Blight, Majors, Rice: "Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells." dans: Journal of virology, Vol. 77, Issue 3, pp. 2029-37, (2003) (PubMed).

Randall, Grakoui, Rice: "Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 235-40, (2003) (PubMed).

Moradpour, Bieck, Hügle, Wels, Wu, Hong, Blum, Bartenschlager: "Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase." dans: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 593-601, (2002) (PubMed).

Détails sur HCV 1b NS5B

Antigène: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Abstract: HCV NS5b Produits

Classe de substances: Viral Protein

Sujet: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.