Cet anticorp Beta-Phosphoglucomutase est non-conjugé
Application
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
Specificité
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified beta-Phosphoglucomutase [Lactococcus lacti]. Cross reactivity against beta-Phosphoglucomutase from other sources is unknown.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 mg ofbeta-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 15,000 of the reconstitution concentration is suggestedfor this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.