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Recombinant eGFP anticorps

eGFP Reactivité: Aequorea victoria ELISA, IP, ICC Hôte: Human Monoclonal G3 unconjugated Recombinant Antibody
N° du produit ABIN1169226
  • Antigène Tous les produits eGFP
    eGFP (Enhanced Green Fluorescent Protein (eGFP))
    Type d'anticorp
    Recombinant Antibody
    Reactivité
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Aequorea victoria
    Hôte
    • 16
    • 5
    • 1
    Human
    Clonalité
    • 16
    • 6
    Monoclonal
    Conjugué
    • 8
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp eGFP est non-conjugé
    Application
    • 21
    • 13
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificité
    Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).
    Purification
    Purified from HEK 293 cell culture supernatant.
    Pureté
    >95 % (SDS-PAGE)
    Immunogène
    Enhanced Green Fluorescent Proteins (EGFP).
    Clone
    G3
    Isotype
    IgG2
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    anti-EGFP, monoclonal antibody (recombinant) (G3) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    Date de péremption
    12 months
  • Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." dans: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, (2009) (PubMed).

  • Antigène
    eGFP (Enhanced Green Fluorescent Protein (eGFP))
    Autre désignation
    EGFP (eGFP Produits)
    Sujet
    Anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
    UniProt
    P42212
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