ASK1 anticorps (pSer83)
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- Antigène Voir toutes ASK1 (MAP3K5) Anticorps
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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Épitope
- AA 76-87, pSer83
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ASK1 est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.
- Purification
- Multi-step process.
- Immunogène
- This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
- Top Product
- Discover our top product MAP3K5 Anticorps primaire
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- Indications d'application
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This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimalreactivity by Western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, Immunohistochemistry andImmunoprecipitation. Recommended Dilutions: For immunoblotting a 1/1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 canbe used as a positive control. For ELISA a 1/5,000-1/10,000 dilution is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 2.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Dilute only prior to immediate use . Avoid cycles of freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- Antigène
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
- Autre désignation
- MEKK5 / ASK1 (MAP3K5 Produits)
- Synonymes
- anticorps ASK1, anticorps MAPKKK5, anticorps MEKK5, anticorps mitogen-activated protein kinase kinase kinase 5, anticorps MUD21.11, anticorps MUD21_11, anticorps 7420452D20Rik, anticorps ASK, anticorps Mekk5, anticorps Ask1, anticorps RGD1306565, anticorps mitogen-activated protein kinase kinase kinase 5, anticorps MAP3K5, anticorps MAPKKK5, anticorps Map3k5, anticorps map3k5
- Sujet
- ASK-1 (apoptosis signal-regulating kinase 1- also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The overall amino acid sequence identity between the mouse and human ASK1 is 91.9 % . ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1). Please refer to the reference list at the end of this document for further information.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
- ID gène
- 4217
- NCBI Accession
- NP_005914
- UniProt
- Q99683
- Pathways
- Signalisation MAPK, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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