IL1R1
Reactivité: Humain
WB, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061). Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Agent conservateur
Azide free
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." dans: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, (1991) (PubMed).
Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, (1989) (PubMed).
The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors. Synonyms: IL-1 Receptor, Type I/p80