IKBKG
Reactivité: Humain
WB, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Volume d'échantillon
5 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Shifera: "The zinc finger domain of IKKγ (NEMO) protein in health and disease." dans: Journal of cellular and molecular medicine, Vol. 14, Issue 10, pp. 2404-14, (2010) (PubMed).
Carter, Pennington, Ungurait, Ballard: "In vivo identification of inducible phosphoacceptors in the IKKgamma/NEMO subunit of human IkappaB kinase." dans: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19642-8, (2003) (PubMed).
Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, (1998) (PubMed).
Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." dans: Nature, Vol. 395, Issue 6699, pp. 297-300, (1998) (PubMed).
DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." dans: Nature, Vol. 388, Issue 6642, pp. 548-54, (1997) (PubMed).
Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." dans: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, (1997) (PubMed).
Antigène
IKBKG
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))
The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKgamma (pS376). IKKgamma is also known an NEMO (NF-kappa-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. Activated NF-kappaB regulates genes involved in various pathways including inflammation, immunity, and cell survival signaling pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK)4 and MEKK1. IKKbeta has been shown to phosphorylate IKKgamma at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia virus type 1. IKKgamma has an essential regulatory role in the IKK complex and is required for the activation of the NF-kappaB pathway in response to multiple cellular stimuli. IKKgamma isoforms migrate as a doublet of ~50-55 kDa upon Western blot analysis. Synonyms: IKBKG, IKKgamma, IKKG, IKK-gamma, AMCBX1, FIP3, IKKAP1, IP1, IP2, IPD2, NEMO