MAPK14
Reactivité: Humain
WB, IHC, IF, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody is conjugated to Pacific Blue™ under optimum conditions, and unreacted Pacific Blue™ was removed.
Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, (1997) (PubMed).
Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." dans: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, (1996) (PubMed).
Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." dans: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, (1994) (PubMed).
Antigène
MAPK14
(Mitogen-Activated Protein Kinase 14 (MAPK14))
Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the Mitogen Activated Protein Kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAPKs include p38alpha (MAPK14), beta (MAPK11), gamma (MAPK12), and delta (MAPK13). These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP Kinase Kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in multiple signal transduction pathways. The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38alpha, beta, gamma, and delta. Synonyms: MK14, 11, 12, 13, CSBP1, SAPK2, 2A, 3, 4, MX12, ERK-6, ERK5