PTPN11
Reactivité: Humain
WB, IF, IP
Hôte: Lapin
Monoclonal
unconjugated
Indications d'application
This antibody conjugate is suitable for intracellular staining of cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Fornasa, Groyer, Clement, Dimitrov, Compain, Gaston, Varthaman, Khallou-Laschet, Newman, Graff-Dubois, Nicoletti, Caligiuri: "TCR stimulation drives cleavage and shedding of the ITIM receptor CD31." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 10, pp. 5485-92, (2010) (PubMed).
MacGillivray, Herrera-Abreu, Chow, Shek, Wang, Vachon, Feng, Siminovitch, McCulloch, Downey: "The protein tyrosine phosphatase SHP-2 regulates interleukin-1-induced ERK activation in fibroblasts." dans: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27190-8, (2003) (PubMed).
Lu, Gong, Bar-Sagi, Cole: "Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling." dans: Molecular cell, Vol. 8, Issue 4, pp. 759-69, (2001) (PubMed).
Bennett, Tang, Sugimoto, Walsh, Neel: "Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 15, pp. 7335-9, (1994) (PubMed).
Ahmad, Banville, Zhao, Fischer, Shen: "A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 6, pp. 2197-201, (1993) (PubMed).
Antigène
PTPN11
(Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
SHP2 is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 reportedly contains two SH2 domains, both of which are N-terminal to the PTP catalytic domain. SH2 PTPs are believed to work in conjunction with protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression. The expression of SHP2 has been reported to be highest in brain, heart, and kidney. SHP2 is phosphorylated at two C-terminal tyrosine residues: Y542 and Y580. Phosphorylation of Y542 creates an interaction in the N-terminal SH2 domain and appears to relieve basal inhibition of the PTP, while phosphorylation at Y580 creates an interaction at the C-terminal that stimulates PTP activity. The L99-921 monoclonal antibody recognizes the phosphorylated Y542 of SHP2 (PTP2C isoform 2). The homologous phosphorylation site in the PTP2Ci splice variant (isoform 1) is Y546. The specificity of this antibody was validated by confirming, using western blot analysis, that RNA-mediated interference (RNAi) of the specific protein was able to down-regulate the expression of SHP2 (pY542). Synonyms: PTN11, PTP1D, PTP2C, PTPN11, SH-PTP2, SH-PTP3, SHP-2, SHPTP2