STAT4
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA, IP, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Volume d'échantillon
5 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Kisseleva, Bhattacharya, Braunstein, Schindler: "Signaling through the JAK/STAT pathway, recent advances and future challenges." dans: Gene, Vol. 285, Issue 1-2, pp. 1-24, (2002) (PubMed).
Visconti, Gadina, Chiariello, Chen, Stancato, Gutkind, OShea: "Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity." dans: Blood, Vol. 96, Issue 5, pp. 1844-52, (2000) (PubMed).
Antigène
STAT4
(Signal Transducer and Activator of Transcription 4 (STAT4))
anticorps SLEB11, anticorps signal transducer and activator of transcription 4, anticorps STAT4, anticorps stat4, anticorps Stat4
Sujet
The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat4 has been shown to play an important role in development of T helper cells, specifically the Th1 subset. Stat4 is activated by IL-12 and by type 1 interferons. Knockout mice supported the role that Stat4 plays in IL-12 signaling because lymphocytes from Stat 4-/- mice could neither differentiate into Th1 cells nor produce IFN-gamma in response to treatment with IL-12. IFN-gamma plays an important role in host defense. A key component in the activation of Stat4 is the phosphorylation on tyrosine and serine residues in response to IL-12 stimulation. IL-12 stimulation leads to the phosphorylation of Stat4 on tyrosine 693 and serine 721. Transcriptional activity of Stat4 has been shown to be significantly reduced when residues Y693 and S721 are mutated. Synonyms: Signal transducer and activator of transcription 4, SLEB11