STAT5A
Reactivité: Humain, Souris, Rat, Chien
WB, IHC, IF, IP
Hôte: Souris
Monoclonal
89-Stat5
unconjugated
Indications d'application
This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD™ Phosflow Fix Buffer I).
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.
Johnston, Choi, Diamond, Yang, Crotty: "STAT5 is a potent negative regulator of TFH cell differentiation." dans: The Journal of experimental medicine, Vol. 209, Issue 2, pp. 243-50, (2012) (PubMed).
Suni, Maino: "Flow cytometric analysis of cell signaling proteins." dans: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, (2011) (PubMed).
Prlic, Bevan: "Exploring regulatory mechanisms of CD8+ T cell contraction." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 43, pp. 16689-94, (2008) (PubMed).
Riou, Yassine-Diab, Van grevenynghe, Somogyi, Greller, Gagnon, Gimmig, Wilkinson, Shi, Cameron, Campos-Gonzalez, Balderas, Kelvin, Sekaly, Haddad: "Convergence of TCR and cytokine signaling leads to FOXO3a phosphorylation and drives the survival of CD4+ central memory T cells." dans: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 79-91, (2007) (PubMed).
Van De Wiele, Marino, Murray, Vo, Whetsell, Teague: "Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, (2004) (PubMed).
Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." dans: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).
Imada, Leonard: "The Jak-STAT pathway." dans: Molecular immunology, Vol. 37, Issue 1-2, pp. 1-11, (2000) (PubMed).
Liu, Gaffen, Goldsmith: "JAK/STAT signaling by cytokine receptors." dans: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, (1998) (PubMed).
Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." dans: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).
Antigène
STAT5A
(Signal Transducer and Activator of Transcription 5A (STAT5A))
anticorps MGF, anticorps STAT5, anticorps AA959963, anticorps Stat5, anticorps STAT5A/MGF, anticorps STATA5, anticorps STAT5A, anticorps stat5, anticorps stat5b, anticorps signal transducer and activator of transcription 5A, anticorps signal transducer and activator of transcription 5a, anticorps STAT5A, anticorps Stat5a, anticorps stat5a, anticorps LOC100348518, anticorps LOC100716811
Sujet
Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699.