Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IL-12 (p70) bioactivity. Neutralization may be measured with a proliferation assay using 200 pg/mL recombinant human IL-12 (p70) (Cat. No. 554613) to stimulate human PBMC as indicator cells (i.e. preincubation of the antibody with recombinant human IL-12 (p70) for 60 minutes). 50% Neutralization (ND50) at 10-300 ng/mL >95% Neutralization at 1-20 µg/mL
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
IL12
Reactivité: Souris
ELISA, ELISpot
Hôte: Rat
Monoclonal
C17-8
unconjugated
Indications d'application
ELISA (IL-12 p70 Detection): The biotinylated C8.6 (Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p70). Biotinylated C8.6 antibody can be paired with the purified 20C2 antibody (Cat. No. 555065) as the capture antibody and recombinant human IL-12 (p70) protein (Cat. No. 554613) as the standard. For testing human IL-12 (p70) in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Human IL-12 (p70) ELISA Set (Cat. No. 555183). ELISA (IL-12 p40 Detection): The biotinylated C8.6 (Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p40). Biotinylated C8.6 antibody can be paired with the purified C8.3 antibody (Cat. No. 551227) as the capture antibody with recombinant human IL-12 (p40) protein (Cat. No. 554633) as the standard. For testing human IL-12 (p40) in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Human IL-12 (p40) ELISA Set (Cat. No. 555171).
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Agent conservateur
Azide free
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." dans: Immunity, Vol. 13, Issue 5, pp. 715-25, (2001) (PubMed).
DAndrea, Aste-Amezaga, Valiante, Ma, Kubin, Trinchieri: "Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells." dans: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 1041-8, (1993) (PubMed).
DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." dans: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).
The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN-gamma induction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23.