Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificité
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Plasminogen (Human Plasma). Cross reactivity against Plasminogen from other sources is unknown.
Suitable for Immunoblotting (Western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Plasminogen [Human Plasma] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitution concentration is suggestedfor this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 With 0.01 % (w/v) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below.
Funk, Schaarschmidt, Slesiona, Hallström, Horn, Brock: "The glycolytic enzyme enolase represents a plasminogen-binding protein on the surface of a wide variety of medically important fungal species." dans: International journal of medical microbiology : IJMM, Vol. 306, Issue 1, pp. 59-68, (2016) (PubMed).
Koenigs, Stahl, Averhoff, Göttig, Wichelhaus, Wallich, Zipfel, Kraiczy: "CipA of Acinetobacter baumannii Is a Novel Plasminogen Binding and Complement Inhibitory Protein." dans: The Journal of infectious diseases, Vol. 213, Issue 9, pp. 1388-99, (2016) (PubMed).
Schott, Grosskinsky, Brenner, Kraiczy, Wallich: "Molecular characterization of the interaction of Borrelia parkeri and Borrelia turicatae with human complement regulators." dans: Infection and immunity, Vol. 78, Issue 5, pp. 2199-208, (2010) (PubMed).