CD8 anticorps
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- Antigène Voir toutes CD8 Anticorps
- CD8
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD8 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- Specificité
- This antibody recognizes Ly2.1 and Ly2.2 cells, reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic.
- Purification
- Affinity Chromatography on Protein A
- Immunogène
- T cell clone, C6. Remarks: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
- Clone
- KT15
- Isotype
- IgG2a
- Top Product
- Discover our top product CD8 Anticorps primaire
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- Indications d'application
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Flow Cytometry: 1/100 - 1/200, use 10 μL of the suggested working dilution to label 10^6cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- CD8
- Autre désignation
- CD8 (CD8 Produits)
- Synonymes
- anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps CD8a molecule, anticorps CD8A
- Sujet
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
- ID gène
- 12525
- NCBI Accession
- NP_001074579
- UniProt
- P01731
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