CD19 anticorps
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- Antigène Voir toutes CD19 Anticorps
- CD19 (CD19 Molecule (CD19))
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD19 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This antibody recognises the CD19 cell surface antigen, a 95 kDa glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
- Purification
- Affinity Chromatography on Protein G
- Immunogène
- Human K562 cell line transfected with Murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
- Clone
- 6D5
- Isotype
- IgG2a
- Top Product
- Discover our top product CD19 Anticorps primaire
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- Indications d'application
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Flow Cytometry: Use 10 μL of 1/25-1/100 diluted antibody to label 10^6 cells in 100 μL. Immonohistochemistry on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- CD19 (CD19 Molecule (CD19))
- Autre désignation
- CD19 (CD19 Produits)
- Sujet
- CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 Molecule is expressed on 100 % of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10 % of normal human peripheral blood cells and approximately 60 % of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12 % of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
- ID gène
- 12478
- NCBI Accession
- NP_033974
- UniProt
- P25918
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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