Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
Specificité
This antibody recognises the CD158b cell surface antigen. This antibody also recognises a 50 kDa molecule, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail.
Purification
Purified
Immunogène
NK cell clone E57. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3UI myeloma cell line.
Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide and 0.1 % Bovine Serum Albumin
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
KIR2DL3
(Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 3 (KIR2DL3))
anticorps CD158B2, anticorps CD158b, anticorps GL183, anticorps KIR-023GB, anticorps KIR-K7b, anticorps KIR-K7c, anticorps KIRCL23, anticorps NKAT, anticorps NKAT2, anticorps NKAT2A, anticorps NKAT2B, anticorps p58, anticorps killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 3, anticorps KIR2DL3
Sujet
CD158b is a 58kD glycoprotein expressed by a subset of NK cells (also known as p58.2 antigen). The antigen is a members of the newly described natural killer cell receptor family. CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. This antibody can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.Synonyms: KIRCL23, Killer cell immunoglobulin-like receptor 2DL3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2