Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Poly-ADP-Ribose anticorps

PAR Reactivité: Humain WB, IHC (p), IHC (fro), IP Hôte: Souris Monoclonal 10H unconjugated
N° du produit ABIN118982
  • Antigène Tous les produits Poly-ADP-Ribose (PAR)
    Poly-ADP-Ribose (PAR)
    Reactivité
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 2
    Souris
    Clonalité
    • 2
    Monoclonal
    Conjugué
    • 2
    Cet anticorp Poly-ADP-Ribose est non-conjugé
    Application
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Réactivité croisée (Details)
    Species reactivity (expected):Broad.
    Species reactivity (tested):Human.
    Purification
    Purified
    Immunogène
    Purified poly (ADP-ribose) polymer, 10-50 unit chain length.
    Clone
    10H
    Isotype
    IgG3
  • Indications d'application
    Immunohistology on frozen sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Immunoprecipitation. Western Blot: 1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, 50 % Glycerol
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    Poly-ADP-Ribose (PAR)
    Abstract
    PAR Produits
    Synonymes
    anticorps 2310036I02Rik, anticorps AW260507, anticorps Emv66, anticorps par, anticorps AFG3-like AAA ATPase 2, anticorps Afg3l2
    Sujet
    PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).Synonyms: PADPR
Vous êtes ici:
Support technique