CCL5 anticorps
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- Antigène Voir toutes CCL5 Anticorps
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CCL5 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- Recognizes Human RANTES chemokine. Other species not tested.
- Purification
- Affinity Chromatography on Protein A.
- Immunogène
- E. Coli derived recombinant Human Rantes (Cat#PA123)
- Clone
- 5B3-D5
- Isotype
- IgG2b
- Top Product
- Discover our top product CCL5 Anticorps primaire
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- Indications d'application
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ELISA: A 1/8,000 dilution of a 1 mg/mL antibody solution will give satisfactory results in a direct ELISA using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories). Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. Also reported to work in: Immunoprecipitation, Immunohistochemistry on Frozen sections and Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile water to a concentration of 1.0 mg/mL.
- Buffer
- PBS without preservatives
- Agent conservateur
- Without preservative
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The lyophilized antibody is stable at room temperature for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water, the antibody is stable for six weeks at 2-8 °C.
- Date de péremption
- 12 months
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- Antigène
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- Autre désignation
- RANTES / CCL5 (CCL5 Produits)
- Synonymes
- anticorps Rantes, anticorps Scya5, anticorps D17S136E, anticorps RANTES, anticorps SCYA5, anticorps SIS-delta, anticorps SISd, anticorps TCP228, anticorps eoCP, anticorps MuRantes, anticorps C-C motif chemokine 5-like, anticorps C-C motif chemokine ligand 5, anticorps chemokine (C-C motif) ligand 5, anticorps LOC100230935, anticorps CCL5, anticorps Ccl5
- Sujet
- RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5. This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1. CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228
- ID gène
- 6352
- NCBI Accession
- NP_002976
- UniProt
- P13501
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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