This antibody recognises an antigen which appears to be identical to the synaptic vesicle protein synaptophysin. It reacts with synaptophysin expressed in CHO cells (3., 8.).
Purification
Gel filtration.
Immunogène
Crude human synaptophysin immunoprecipitate. Spleen cells from immunised BALB/c mice were fused with cells of the NSO mouse myeloma cell line.
ELISA. Western Blot: 1/500. Immunohistochemistry on Paraffin Sections: 1/25. Recommended Positive Control: Pancreas. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.