CD3 anticorps (Cytoplasmic Domain)
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- Antigène Voir toutes CD3 Anticorps
- CD3
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Épitope
- Cytoplasmic Domain
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Reactivité
- Humain, Souris, Poulet, Chien, Porc, Cheval
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD3 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Séquence
- Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
- Specificité
- This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).
- Réactivité croisée (Details)
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Species reactivity (expected):Mammals, Birds.
Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena. - Purification
- Affinity Chromatography on Protein G
- Immunogène
- Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
- Clone
- CD3-12
- Isotype
- IgG1
- Top Product
- Discover our top product CD3 Anticorps primaire
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- Indications d'application
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Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store the reconstituted antibody undiluted at 2-8 °C.
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Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." dans: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).
: "Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control." dans: Cancer research, Vol. 71, Issue 22, pp. 6997-7009, (2011) (PubMed).
: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).
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Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." dans: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).
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- Antigène
- CD3
- Autre désignation
- CD3 (CD3 Produits)
- Synonymes
- anticorps CD3, anticorps AI504783, anticorps CD3epsilon, anticorps T3e, anticorps cd3-gamma, anticorps cd3g, anticorps cd3g-a, anticorps cd3g/d, anticorps cd3gd, anticorps t3g, anticorps CD3e molecule, anticorps CD3d molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps CD3g molecule S homeolog, anticorps CD3E, anticorps CD3D, anticorps Cd3e, anticorps cd3g.S
- Sujet
- T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
- ID gène
- 916
- NCBI Accession
- NP_000724
- UniProt
- P07766
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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