Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
Specificité
This antibody recognizes CD177 (Neutrophil Glycoprotein NB1). Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). MEM-166 recognizes CD177 under Non-Reducing Conditions.
Flow cytometry (1/50 - 1/100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western blot: Non-reducing conditions. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97 % of the caucasian population.Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1