This antibody recognises KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family, CD158a, CD158b and P50.3. Clone NKVFS1 specifically recognises the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). The Clone is reported to have Functional Activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot. Functional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, None, None
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.Synonyms: CD158a, CD158b, CD158i, KI2DL1, KILLER CELL Immunoglobin-Like Receptors, KIR2DL3, KIR2DS4